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Mod-26 Lec-26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays
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Indian Institute of Technology Bombay
Biotechnology - Proteomics: Principles and Techniques
Mod-26 Lec-26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays
Course Lectures
Mod-01 Lec-01 Introduction to Proteomics Course
Prof. Sanjeeva Srivastava
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Mod-02 Lec-02 Central Dogma: Basics of DNA, RNA, Proteins
Prof. Sanjeeva Srivastava
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Mod-03 Lec-03 Genomics and Transcriptomics: Why proteomics?
Prof. Sanjeeva Srivastava
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Mod-04 Lec-04 Proteins: Amino acids and structural levels of proteins
Prof. Sanjeeva Srivastava
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Mod-05 Lec-05 Proteins: Folding and misfolding
Prof. Sanjeeva Srivastava
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Mod-06 Lec-06 Protein Purification and Peptide Isolation using Chromatography
Prof. Sanjeeva Srivastava
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Mod-07 Lec-07 Enzymes: Basic concepts, Catalytic and Regulatory strategies
Prof. Sanjeeva Srivastava
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Mod-08 Lec-08 Proteomics and Systems Biology
Prof. Sanjeeva Srivastava
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Mod-09 Lec-09 Sample preparation for proteomics applications
Prof. Sanjeeva Srivastava
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Mod-10 Lec-10 Sample preparation for proteomics applications: Serum and bacterial proteome
Prof. Sanjeeva Srivastava
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Mod-11 Lec-11 Sample preparation for proteomics applications
Prof. Sanjeeva Srivastava
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Mod-12 Lec-12 Gelbased Proteomics
Prof. Sanjeeva Srivastava
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Mod-13 Lec-13 Gel-based Proteomics Two-dimensional electrophoresis(continued)
Prof. Sanjeeva Srivastava
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Mod-14 Lec-14 Gel-based Proteomics Two-dimensional electrophoresis Workflow
Prof. Sanjeeva Srivastava
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Mod-15 Lec-15 Two-dimensional electrophoresis: Image processing and data analysis
Prof. Sanjeeva Srivastava
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Mod-16 Lec-16 Two-dimensional difference gel electrophoresis (2D DIGE)
Prof. Sanjeeva Srivastava
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Mod-17 Lec-17 Difference gel electrophoresis (continued) Discussion and data analysis
Prof. Sanjeeva Srivastava
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Mod-18 Lec-18 Applications of two dimensional electrophoresis
Prof. Sanjeeva Srivastava
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Mod-19 Lec-19 Applications of 2-DE and DIGE
Prof. Sanjeeva Srivastava
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Mod-20 Lec-20 Fundamentals of mass spectrometry
Prof. Sanjeeva Srivastava
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Mod-21 Lec-21 Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF)
Prof. Sanjeeva Srivastava
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Mod-22 Lec-22 Liquid chromatography-Mass spectrometry (LC-MS/MS)
Prof. Sanjeeva Srivastava
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Mod-23 Lec-23 Hybrid-MS/MS Configurations
Prof. Sanjeeva Srivastava
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Mod-24 Lec-24 Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell Culture(SILAC)
Prof. Sanjeeva Srivastava
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Mod-25 Lec-25 Quantitative Proteomics: iTRAQ and TMT
Prof. Sanjeeva Srivastava
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Mod-26 Lec-26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays
Prof. Sanjeeva Srivastava
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Mod-27 Lec-27 Microarray workflow: Label-based detection techniques
Prof. Sanjeeva Srivastava
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Mod-28 Lec-28 Microarray related concepts: Recombinational cloning Cell-free expression
Prof. Sanjeeva Srivastava
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Mod-29 Lec-29 Cell-free synthesis based protein microarrays
Prof. Sanjeeva Srivastava
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Mod-30 Lec-30 Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array
Prof. Sanjeeva Srivastava
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Mod-31 Lec-31 Microarray work-flow: Image scanning and processing
Prof. Sanjeeva Srivastava
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Mod-32 Lec-32 Microarray work-flow: Data analysis
Prof. Sanjeeva Srivastava
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Mod-33 Lec-33 Applications of protein microarrays
Prof. Sanjeeva Srivastava
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Mod-34 Lec-34 Applications of cell free protein microarrays
Prof. Sanjeeva Srivastava
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Mod-35 Lec-35 Label-free techniques: SPR and SPRi
Prof. Sanjeeva Srivastava
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Mod-36 Lec-36 Label-free techniques: SPRi, Ellipsometry, Interference
Prof. Sanjeeva Srivastava
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Mod-37 Lec-37 Surface Plasmon Resonance: Biacore SPR and data analysis
Prof. Sanjeeva Srivastava
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Mod-38 Lec-38 Nanotechniques in proteomics
Prof. Sanjeeva Srivastava
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Mod-39 Lec-39 Detection System: Diffraction-based biosensors
Prof. Sanjeeva Srivastava
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Mod-40 Lec-40 Proteomics: Advances and Challenges
Prof. Sanjeeva Srivastava
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