Mod-06 Lec-06 Protein Purification and Peptide Isolation using Chromatography

Course Lectures

• Mod-01 Lec-01 Introduction to Proteomics Course

  Prof. Sanjeeva Srivastava

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• Mod-02 Lec-02 Central Dogma: Basics of DNA, RNA, Proteins

  Prof. Sanjeeva Srivastava

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• Mod-03 Lec-03 Genomics and Transcriptomics: Why proteomics?

  Prof. Sanjeeva Srivastava

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• Mod-04 Lec-04 Proteins: Amino acids and structural levels of proteins

  Prof. Sanjeeva Srivastava

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• Mod-05 Lec-05 Proteins: Folding and misfolding

  Prof. Sanjeeva Srivastava

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• Mod-06 Lec-06 Protein Purification and Peptide Isolation using Chromatography

  Prof. Sanjeeva Srivastava

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• Mod-07 Lec-07 Enzymes: Basic concepts, Catalytic and Regulatory strategies

  Prof. Sanjeeva Srivastava

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• Mod-08 Lec-08 Proteomics and Systems Biology

  Prof. Sanjeeva Srivastava

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• Mod-09 Lec-09 Sample preparation for proteomics applications

  Prof. Sanjeeva Srivastava

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• Mod-10 Lec-10 Sample preparation for proteomics applications: Serum and bacterial proteome

  Prof. Sanjeeva Srivastava

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• Mod-11 Lec-11 Sample preparation for proteomics applications

  Prof. Sanjeeva Srivastava

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• Mod-12 Lec-12 Gelbased Proteomics

  Prof. Sanjeeva Srivastava

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• Mod-13 Lec-13 Gel-based Proteomics Two-dimensional electrophoresis(continued)

  Prof. Sanjeeva Srivastava

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• Mod-14 Lec-14 Gel-based Proteomics Two-dimensional electrophoresis Workflow

  Prof. Sanjeeva Srivastava

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• Mod-15 Lec-15 Two-dimensional electrophoresis: Image processing and data analysis

  Prof. Sanjeeva Srivastava

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• Mod-16 Lec-16 Two-dimensional difference gel electrophoresis (2D DIGE)

  Prof. Sanjeeva Srivastava

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• Mod-17 Lec-17 Difference gel electrophoresis (continued) Discussion and data analysis

  Prof. Sanjeeva Srivastava

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• Mod-18 Lec-18 Applications of two dimensional electrophoresis

  Prof. Sanjeeva Srivastava

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• Mod-19 Lec-19 Applications of 2-DE and DIGE

  Prof. Sanjeeva Srivastava

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• Mod-20 Lec-20 Fundamentals of mass spectrometry

  Prof. Sanjeeva Srivastava

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• Mod-21 Lec-21 Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF)

  Prof. Sanjeeva Srivastava

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• Mod-22 Lec-22 Liquid chromatography-Mass spectrometry (LC-MS/MS)

  Prof. Sanjeeva Srivastava

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• Mod-23 Lec-23 Hybrid-MS/MS Configurations

  Prof. Sanjeeva Srivastava

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• Mod-24 Lec-24 Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell Culture(SILAC)

  Prof. Sanjeeva Srivastava

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• Mod-25 Lec-25 Quantitative Proteomics: iTRAQ and TMT

  Prof. Sanjeeva Srivastava

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• Mod-26 Lec-26 Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays

  Prof. Sanjeeva Srivastava

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• Mod-27 Lec-27 Microarray workflow: Label-based detection techniques

  Prof. Sanjeeva Srivastava

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• Mod-28 Lec-28 Microarray related concepts: Recombinational cloning Cell-free expression

  Prof. Sanjeeva Srivastava

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• Mod-29 Lec-29 Cell-free synthesis based protein microarrays

  Prof. Sanjeeva Srivastava

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• Mod-30 Lec-30 Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array

  Prof. Sanjeeva Srivastava

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• Mod-31 Lec-31 Microarray work-flow: Image scanning and processing

  Prof. Sanjeeva Srivastava

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• Mod-32 Lec-32 Microarray work-flow: Data analysis

  Prof. Sanjeeva Srivastava

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• Mod-33 Lec-33 Applications of protein microarrays

  Prof. Sanjeeva Srivastava

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• Mod-34 Lec-34 Applications of cell free protein microarrays

  Prof. Sanjeeva Srivastava

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• Mod-35 Lec-35 Label-free techniques: SPR and SPRi

  Prof. Sanjeeva Srivastava

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• Mod-36 Lec-36 Label-free techniques: SPRi, Ellipsometry, Interference

  Prof. Sanjeeva Srivastava

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• Mod-37 Lec-37 Surface Plasmon Resonance: Biacore SPR and data analysis

  Prof. Sanjeeva Srivastava

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• Mod-38 Lec-38 Nanotechniques in proteomics

  Prof. Sanjeeva Srivastava

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• Mod-39 Lec-39 Detection System: Diffraction-based biosensors

  Prof. Sanjeeva Srivastava

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• Mod-40 Lec-40 Proteomics: Advances and Challenges

  Prof. Sanjeeva Srivastava

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